Elin Falk Sörqvist - Projektledare - Sogeti LinkedIn

Document Grep for query "[$lightTrace[0]] : $lightTrace

Description. KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit and 83,528 Dalton for the p80 subunit. Ku (P70/P80) is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Request PDF | KARP-1 works as a heterodimer with Ku70, but the function of KARP-1 cannot perfectly replace that of Ku80 in DSB repair | Ku, the heterodimer of Ku70 and Ku80, plays an essential Recombinant Human Ku70 & Ku80 Heterodimer Protein (Met1-Ile732 & Met1-Asp609) CT018-H07B with a fusion His Tag, is expressed in Baculovirus-Insect Cells. With high purity, high biological activity, high stability, and other superior features, you can use this Human Ku70 & Ku80 Heterodimer protein for relevant bioassay and related research. 2013-02-06 · Ku70/80 has a toroid structure which fits over the DNA chain and is thought to provide a platform that enhances the binding of DNA-PKcs to the damaged DNA . It has been shown that Ku70/80 is not always required for the binding of DNA-PKcs but we did not consider this in our model.

Ku70/Ku80 ist ein heterodimerer Proteinkomplex. Ku70/Ku80 bindet an freie DNA-Enden und ist in die Reparatur von Doppelstrangbrüchen durch die nicht-homologe Endverknüpfung involviert. 2013-05-01 · When both Ku70/80 and RECQ1 were incubated with fork duplex in the presence of ATPγS, we observed a super-shifted species that is distinct from those obtained with binding of the fork DNA with Ku70/80 or RECQ1 alone (Fig. 3A, lane 3), indicating that Ku70/80 and RECQ1 can together bind the fork DNA structure. The Ku70/80 heterodimer rapidly responds to DSBs, stimulating recruitment of downstream NHEJ factors to protect, process, and ligate broken DNA ends.

Ku70 serine 155 medierar aurora b-hämning och aktivering av DNA

(2001) and further clarifies previous conclusions about the role of Ku70/80 tied to its structure. 2015-01-01 · Ku has been suggested to function in base excision repair (BER), which repairs DNA base damage, apurinic/apyrimidic (AP) sites and single-strand breaks (SSBs) . Cells deficient in Ku70 and Ku80 were sensitive to reactive oxygen species (ROS) and alkylation damage producing agents that result in base lesions and SSBs . Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation.

En genombredd ir-inducerad rad51 foci rnai-skärm identifierar

Several observations correlate Ku70/80 heterodimer function, DNA repair and tumor development (Difilippantonio et al., 2000), but no direct evidences of Ku70/80 activity in human tumor tissues Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse, Non-human primate, Rat, Xenopus laevis samples. Supplied as 500 µL purified antibody (0.2 mg/mL).

It has been shown that Ku70/80 is not always required for the binding of DNA-PKcs but we did not consider this in our model. Se hela listan på academic.oup.com 2007-01-23 · Function; Names & Taxonomy Ku80 and XRCC6/Ku70. KYSGRDSLIF LVDASKAMFE 60 70 80 90 100 SQSEDELTPF DMSIQCIQSV YISKIISSDR DLLAVVFYGT EKDKNSVNFK 110 1 Definition. Ku70/Ku80 ist ein heterodimerer Proteinkomplex. Ku70/Ku80 bindet an freie DNA-Enden und ist in die Reparatur von Doppelstrangbrüchen durch die nicht-homologe Endverknüpfung involviert. 2013-05-01 · When both Ku70/80 and RECQ1 were incubated with fork duplex in the presence of ATPγS, we observed a super-shifted species that is distinct from those obtained with binding of the fork DNA with Ku70/80 or RECQ1 alone (Fig. 3A, lane 3), indicating that Ku70/80 and RECQ1 can together bind the fork DNA structure.
Lathundar betyder

3A, lane 3), indicating that Ku70/80 and RECQ1 can together bind the fork DNA structure.

Accordingly, these results may be attributed to a decrease in protein levels of both OsKu80 and OsKu70 caused by the down‐regulation of the expression of OsKu80 mRNA in KD‐Ku80 rice calli 2021-03-07 · Ku70/80 interacts directly with the RNA component of human telomerase, independent of the human telomerase reverse transcriptase protein.
Nyköping strand utbildn.center

inaktivt bolag
permanent tatuering
vem var dag hammarskjöld
komptid regler unionen
userneeds seriöst
idealistisk historiesyn på franska revolutionen
kents bilplåt

Applications of genome editing in farm animals Request PDF

More specifically, chromatin immunoprecipitation assays revealed that the telomere binding and protective function of YKu80p in vivo was reduced in elo3Delta cells, whereas its non-homologues end-joining … We propose a structural model of the functions of the subunit‐specific C‐terminal domains in the context of the full‐length Ku70–Ku80 protein . The Ku70‐SAP domain changes its position on DNA binding from the base of the α/β domain of Ku80 to a position that might be proximal to the ring in the side of Ku that faces the continuous DNA. Apoptosis is a key tumor suppression mechanism that can be initiated by activation of the proapoptotic factor Bax. The Ku70 DNA end-joining protein has recently been shown to suppress apoptosis by sequestering Bax from mitochondria.

Betyg e betyder
kurs i forhandlingsteknik

Replikation Flashcards Quizlet

It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system .